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legendplex data analysis software suire  (Qognit Inc)

 
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    Qognit Inc legendplex data analysis software suire
    Legendplex Data Analysis Software Suire, supplied by Qognit Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/legendplex+data+analysis+software/analysis+based+cloud+data+legendplex+software/pm42270592-345-5-11
    Average 86 stars, based on 1 article reviews
    legendplex data analysis software suire - by Bioz Stars, 2026-10
    86/100 stars

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    Software:

    Article Title: Single-cell and spatial transcriptomic analyses of gene therapy-associated retinal inflammation in non-human primates
    Article Snippet: Samples were run on the Cytek Aurora’s 5-Laser Full Spectrum Cytometer 16UV-16V-14B-10YG-8R (Fremont, CA, USA), set up as per the manufacturer’s instructions to gate for the specified bead populations ( www.biolegend.com/legendplex ). .. Data were analyzed in the LEGENDplex Data Analysis Software (Qognit, BioLegend). ..

    Article Title: Immunogenicity and protective efficacy of an intranasal neuraminidase-based influenza vaccine with bacterial cell membrane-derived adjuvants.
    Article Snippet: .. Data were collected using an Attune flow cytometer (Thermo Fisher Scientific) and analyzed with LEGENDplex data analysis software (https://legendplex. qognit.com). .. Group comparisons were performed using one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons in RStudio software (R version 4.3.3, RStudio Inc).

    Article Title: Platelet-Derived Microvesicles Modulate Cytokine and Lipid Mediator Profiles in THP-1 Monocytes and Macrophages.
    Article Snippet: Cytokine release was measured using flow cytometry with the LEGENDplex Human Inflammation Panel 1 (BioLegend), which allows the quantification of IL-1β, interferon (IFN)-α2, IFN-γ, tumor necrosis factor (TNF)-α, macrophage chemoattractant protein (MCP)-1, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-23 and IL-33, following the manufacturer’s protocol. .. The results were analyzed using the cloud-based LEGENDplex data analysis software (Qognit). .. Samples were initially fixed in 2.5% glutaraldehyde (Sigma-Aldrich) in PBS at 4°C for 2 hours, followed by post-fixation in 1% osmium tetroxide (OsO4; Sigma-Aldrich) at 4°C for an additional 2 hours.

    Article Title: Effects of sex and obesity on immune checkpoint inhibition-related cardiac systolic dysfunction in aged mice.
    Article Snippet: In our B-cell mix and match panel we measured the following targets on Cytek Northern Lights flow cytometer: IL-2, IL-6, IL-10, TNFα, TGFβ, IFNγ. .. The concentrations were calculated using LEGENDplex Data Analysis software by Qognit. ..

    Article Title: Increased functional potency of multi-edited CAR-T cells manufactured by a non-viral transfection system
    Article Snippet: .. Flow cytometry standard files were exported for analysis using the LEGENDplex data analysis software Qognit. .. Results were analyzed in Microsoft Excel and graphed using GraphPad Prism (version 9.0).

    Article Title: IL-4R and CXCR2 Contribute to Downregulating Neutrophil-Mediated Response in the Early Stage of Fungal Extract-Induced Allergic Airway Inflammation.
    Article Snippet: .. Analysis was performed using the MACSQuant Analyzer 10 (Miltenyi Biotec) and LEGENDplex Data Analysis Software Qognit, Version 2024-06-15. ..

    Article Title: Cimicifuga racemosa extract Ze 450 shifts macrophage immunometabolism and attenuates pro-inflammatory signaling.
    Article Snippet: Cytokine secretion levels of macrophages were assessed with a Luminex Guava EasyCyte flow cytometer by exiting the samples with a 488 nm blue and a 635 nm red laser and capturing emission wavelengths of 532 nm and 660 nm when acquiring 5000 beads/sample. .. Data analysis was proceeded by using the online available LEGENDplex Data Analysis Software (Qognit, Inc., San Carlos, CA, USA). .. OCR and ECAR were examined using the XF96 Seahorse Metabolic Flux Analyzer from Seahorse Biosciences (Agilent Technologies, Waldbronn, Germany).

    Article Title: Monocyte transcriptome signatures of inflammation and enhanced neutrophil recruitment characterize immunopathology in the blood of TB patients.
    Article Snippet: Samples were washed and analyzed with a BD LSR Fortessa flow cytometer (BD Biosciences). .. Data were analyzed using LEGENDplex Data Analysis Software (Qognit. ..

    Flow Cytometry:

    Article Title: Immunogenicity and protective efficacy of an intranasal neuraminidase-based influenza vaccine with bacterial cell membrane-derived adjuvants.
    Article Snippet: .. Data were collected using an Attune flow cytometer (Thermo Fisher Scientific) and analyzed with LEGENDplex data analysis software (https://legendplex. qognit.com). .. Group comparisons were performed using one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons in RStudio software (R version 4.3.3, RStudio Inc).

    Article Title: Increased functional potency of multi-edited CAR-T cells manufactured by a non-viral transfection system
    Article Snippet: .. Flow cytometry standard files were exported for analysis using the LEGENDplex data analysis software Qognit. .. Results were analyzed in Microsoft Excel and graphed using GraphPad Prism (version 9.0).



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    Legendplex Data Analysis Software Suire, supplied by Qognit Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the <t>LegendPlex</t> NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.
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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
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    legendplex data analysis software suite  (Qognit Inc)
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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
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    legendplex data analysis software v.8.0  (Vigene Biosciences)
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    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by <t>LEGENDplex.</t> Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.
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    Image Search Results


    Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the LegendPlex NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.

    Journal: Molecular Therapy Advances

    Article Title: Single-cell and spatial transcriptomic analyses of gene therapy-associated retinal inflammation in non-human primates

    doi: 10.1016/j.omta.2026.201726

    Figure Lengend Snippet: Cytokine expression profile after AAV gene therapy in NHPs Quantification of vitreous cytokines in NHP1 (A) and NHP2 (B) using the LegendPlex NHP Inflammation Panel with levels expressed as fold-change (log 2) in mean fluorescence intensity (MFI) relative to baseline. RE, right eyes; LE, left eye. (C and D) Single-cell transcriptomic analysis of cytokine gene expression among retinal cell types in NHP1 and NHP2, respectively.

    Article Snippet: Data were analyzed in the LEGENDplex Data Analysis Software (Qognit, BioLegend).

    Techniques: Expressing, Fluorescence, Single Cell, Gene Expression

    Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Journal: Science immunology

    Article Title: Human LFA-1 governs T cell immune surveillance of the skin

    doi: 10.1126/sciimmunol.adz8360

    Figure Lengend Snippet: Proliferation of T cells from patients ( N = 6), TC ( N = 8), and local controls (LC; N = 6), assessed by CFSE dilution after 5 days of anti-CD3/CD28 stimulation. ( B ) Cytokine production by memory T cells from patients ( N = 6), TC ( N = 8), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, assessed by flow cytometry. ( C ) Cytokine production by CLA + (skin-tropic) and CLA − memory CD4 T cells from patients ( N = 6) and controls ( N = 14). Cytokines secreted by PBMCs from patients ( N = 6), TC ( N = 6), and LC ( N = 6) after 24-hour anti-CD3/CD28 stimulation, measured by LEGENDplex. Proliferation of αL-deficient T cells (from patients) cocultured with allogeneic MDDCs at the indicated ratios. Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing (LC, N = 10; TC, N = 8; P, N = 6). See also ( to ). Proliferation of allogeneic T cells (from controls, N = 7) induced by MDDCs from P1 or healthy donors (HDs). Representative CFSE histograms are shown on the right (DC:T 1:2). Each dot represents one allogeneic pairing. See also ( and ). Two-way ANOVA tests with Tukey’s correction were used in (A), (B), (D), and (E). Mann-Whitney tests with Holm-Šidák correction were used in (C) and (F). * P < 0.05 and ** P < 0.01. MHC, major histocompatibility complex.

    Article Snippet: Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (Qognit).

    Techniques: Flow Cytometry, MANN-WHITNEY, Immunopeptidomics